calibration curve

Copper Contributor

pls can you kindly put me through on how to use excel to calculate calibration curve

3 Replies
best response confirmed by Hans Vogelaar (MVP)


The process to create a calibration curve in Excel is consistent across different versions, including Excel 365. A calibration curve in Excel is used to relate a measured quantity to its concentration. Here is a basic outline on how to set up a calibration curve using Excel 365:

Step 1: Prepare Your Data

  • Organize your known concentrations in one column and their respective measured values in another.

Step 2: Plotting the Calibration Curve

  • Select your data pairs (concentrations and measured values).
  • Go to the "Insert" tab.
  • Choose "Charts" or "Recommended Charts" and create a scatter plot.

Step 3: Trendline and Equation

  • Click on any data point in the plot to select the data series.
  • Under the "Chart Tools" section, go to "Design" and then "Add Chart Element."
  • Choose "Trendline" and select the appropriate type (linear, polynomial, etc.).

Step 4: Using the Calibration Curve

  • Display the equation and R-squared value on the chart to calculate unknown concentrations from their measured values.

Additional Tips:

  • Always ensure that the trendline and equation align well with your data.
  • Label your axes appropriately for easy understanding of the concentration and measured values.

The steps might differ slightly in their sequence due to interface changes, but the functionalities for creating charts, trendlines, and displaying the equation remain consistent in Excel 365. The text and steps were edited with the help of AI.


Additional info/links:

Generating calibration curve in MS Excel

Worksheets for Analytical Calibration Curves


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Hope this will help you.

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Thank you for your response.

It has really helped.@NikolinoDE