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    1. If you included B1:F1 in the input range, did you specify that the first row contains labels? Otherwise, Excel will treat B1:F1 as data, causing the error that you mention.
    2. Make sure that the empty-looking cells are really empty - try clearing them. If they contain spaces or formulas that return "", you'd get the error message.

    ANOVA can handle really blank cells:

     

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