mthomas1217 You could remove the room from the roomlist (although the room is still technically bookable it will just be harder to find). To do this you can use Remove-DistributionGroupMember:
Remove-DistributionGroupMember -Identity "RoomList" -Member "Room 101"
https://docs.microsoft.com/en-us/powershell/module/exchange/remove-distributiongroupmember?view=exchange-ps
As KarriS mentioned, one option is to use Set-CalendarProcessing to reject booking attempts and then use Set-Mailbox to configure a MailTip:
Set-CalendarProcessing -Identity "Room 101" -AllBookInPolicy $False -AllRequestInPolicy $False
Set-Mailbox -Identity "Room 101" -MailTip "Rooms are currently unavailable"
https://docs.microsoft.com/en-us/powershell/module/exchange/set-calendarprocessing?view=exchange-ps
You could also block book the meeting out in Outlook so it shows as unavailable and rejects all conflicts.