Getting Error while create the database copy.

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The seeding operation failed. Error: An error occurred while running prerequisite checks. Error: The specified database isn't configured for replication and therefore cannot be used to perform seed operations. 
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@Harpreet_Singh  Whats the current status of that database copy on that server? 

Get-MailboxDatabaseCopyStatus

Generally you can just update it to fix 

If its not suspended or throws an error that it needs to be suspended use  Suspend-MailboxDatabaseCopy

then:

Update-MailboxDatabaseCopy  <DB01\Server> -DeleteExistingFiles