Can an MVC 5 application (deployed on Azure) have authentication like windows authentication?

We are planning to implement our enterprise application as an MVC 5 application (intranet site), which will be deployed on Azure. We do not want any sort of login page. We did some research about Azure Active directory, but we are not sure whether following expectation will be meet if we enable Azure Active Directory and sync that with on premise AAD. Will someone please answer whether this would be possible:

User logs in to computer with his/her user ID and password. H/she open the browser and attempts to go to MVC 5 app on Azure. Does s/he has to enter user id/password again or will s/he be able to land on the landing page?

Please suggest. Thank you.